Essential data of the biological examples are:
Typical set up of a test: B1:
Action Against Anthonomus grandis, Spodoptera spp, Heliothis spp.
Young transgenic cotton which express the delta(?WdL)-endotoxin CryIIIA
are sprayed with an aqueous emulsion spray mixture comprising 100, 50,
10, 5, 1 ppm Imidacloprid respectively. After the spray coating has
dried on the cotton plants are populated with 10 adult Anthonomus
grandis, 10 Spodoptera littorales larvae or 10 Heliothis virescens
larvae respectively and introduced into a plastic container. Evaluation
takes place 3 to 10 days later. The percentage reduction in population,
or the percentage reduction in feeding damage (% action), is determined
by comparing the number of dead beetles and the feeding damage to larvae
on the transgenic cotton plants with that of non-transgenic cotton
plants which have been treated with an emulsion spray mixture comprising
Imidacloprid and conventional CryIIIA-toxin at a concentration of in
each case 100, 50, 10, 5, 1 ppm respectively.
In the test the control of the tested insects in the transgenic plant is
superior, while it is insufficient in the non-transgenic plant.
B2-B6 are variations of the same set-up, conclusion the same.
B7: Action Against Ostrinia nubilalis, Spodoptera spp, Heliothis spp.
A plot (a) planted with maize cv KnockOut(r) and an adjacent plot (b) of
the same size which is planted with conventional maize, both showing
natural infestation with Ostrinia nubilalis, Spodoptera or Heliothis,
are sprayed with an aqueous emulsion spray mixture comprising 200, 100,
50, 10, 5, 1 ppm of Ti-435. Immediately afterwards plot (b) is treated
with an emulsion spray mixture comprising 200, 100, 50 ,10, 5, 1 ppm of
the endotoxin expressed by KnockOut(r). Evaluation takes place 6 days
later. The percentage reduction in population (% action) is determined
by comparing the number of dead pests on the plants of plot (a) with
that on the plants of plot (b).
Improved control of Ostrinia nubilalis, Spodoptera spp. or Heliothis
spp. is observed on the plants of plot(a) while plot(b) shows a control
level of not more than 60%.
B8 - B22 are variations with dose up to 400 ppm.
Two tests are with pots placed in an aqueous emulsion. Some tests of 21
Mainly cotton, rice and maize plants. 4 different insects.
in part VI: Synergy and the Advantages.
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